Difference between revisions of "Part:BBa K2595007"
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<partinfo>BBa_K2595007 short</partinfo> | <partinfo>BBa_K2595007 short</partinfo> | ||
| − | - | + | RprA is a small regulatory RNA that is required for wild-type production of RpoS (RNA polymerase, sigma S) [1]. Binding of RprA to Hfq is required for stability and activity in vivo; it binds upstream of the base pairing region within rpoS mRNA [3]. |
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| + | RprA is also involved in negative regulation of biofilm formation (by repressing expression of CsgD). It does so through direct binding of RprA to the 5'-untranslated and translational initiation regions of csgD mRNA, inhibiting translation and promoting csgD mRNA degradation [5]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This synthetic version of RprA follow the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RprA can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2595007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2595007 SequenceAndFeatures</partinfo> | ||
Revision as of 13:40, 17 October 2018
RprA
RprA is a small regulatory RNA that is required for wild-type production of RpoS (RNA polymerase, sigma S) [1]. Binding of RprA to Hfq is required for stability and activity in vivo; it binds upstream of the base pairing region within rpoS mRNA [3].
RprA is also involved in negative regulation of biofilm formation (by repressing expression of CsgD). It does so through direct binding of RprA to the 5'-untranslated and translational initiation regions of csgD mRNA, inhibiting translation and promoting csgD mRNA degradation [5].
Usage and Biology
This synthetic version of RprA follow the same principles as its wild type partner to down-regulate gene expression. This modified construct, however, can target any gene of interest due to its sequence which contains a BaeI restriction enzyme site. After digestion with this restriction enzyme and ligation with a target sequence, RprA can down-regulate the expression of the gene that contains the complementary regions for its targeting sequence. Ligation with the desired targeting sequence does not create any scar in the sRNA sequence, therefore allowing its use for a broad range of gene targets as well as with any biobricks that may benefit from its functions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]