Difference between revisions of "Part:BBa K2586020"

Latest revision as of 13:44, 12 October 2018

AroA*: Resistant aroA-variant in Escherichia coli

This part encodes a resistant variant to the native aroA gene of Escherichia coli. The resistance occurs due to 2 point mutations within the DNA sequence. At position 287 a Guanin was changed to a Cytosin and at position 547 a Guanin was changed to an Adenin.

This part can be used to confer glyphosate resistance to bacteria, as the mutation decreases the interaction of the herbicide with the EPSP-Synthase, which is encoded by the native aroA gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 346
Illegal AgeI site found at 787
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1021

aroA: 3-phosphoshikimate 1-carboxyvinyltransferase

This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase. The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. Three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr. aroA is expressed in the E.coli wildtype.

aroA encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of aroE/aroA allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications. If exposed to glyphosate, E.coli mutants will make changes in the original sequence of aroA to avoid the lethal effects of the substance.

Fig. 1.Crystal structure of AroA

Locus	BSU29750
Isoelectric point	5.34
Molecular weight	39.38 kDa
Protein length	358 aa
Gene length	1074 bp
Function	biosynthesis of aromatic amino acids
Product	3-deoxy-D-arabino-heptulosonate 7-phosphate synthase/ chorismate mutase-isozyme 3
Essential	no
Synonyms	aroG

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2586020 short</partinfo>
-This part encodes a resistant variant to the native <i>aroA</i> gene in <i>Escherichia coli</i>.
+This part encodes a resistant variant to the native <i>aroA</i> gene of <i>Escherichia coli</i>.
 The resistance occurs due to 2 point mutations within the DNA sequence. At position 287 a Guanin was changed to a Cytosin and at position 547 a Guanin was changed to an Adenin.