Difference between revisions of "Part:BBa K2549016"
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This part is one of our SynNotch receptors<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. LaG17 ([[Part:BBa_K2549004]]) is used as the extracellular sensor module to receive the signal input from GFP. mN1c ([[Part:BBa_K2549006]]) is served as the transmembrane core domain of SynNotch, which is evident to have a low basal expression and a high activation efficiency. tTAA ([[Part:BBa_K2446057]]) is an improved tetracycline-controlled transcription activator, which is cleaved after SynNotch activation and drives the expression of the amplifier. Besides, a CD8α signal peptide ([[Part:BBa_K2549044]]) and a Myc-tag ([[Part:BBa_K823036]]) are added to the N terminal of LaG17([[Part:BBa_K2549004]]) for membrane targeting and easy determination of surface expression. | This part is one of our SynNotch receptors<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. LaG17 ([[Part:BBa_K2549004]]) is used as the extracellular sensor module to receive the signal input from GFP. mN1c ([[Part:BBa_K2549006]]) is served as the transmembrane core domain of SynNotch, which is evident to have a low basal expression and a high activation efficiency. tTAA ([[Part:BBa_K2446057]]) is an improved tetracycline-controlled transcription activator, which is cleaved after SynNotch activation and drives the expression of the amplifier. Besides, a CD8α signal peptide ([[Part:BBa_K2549044]]) and a Myc-tag ([[Part:BBa_K823036]]) are added to the N terminal of LaG17([[Part:BBa_K2549004]]) for membrane targeting and easy determination of surface expression. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --!> |
===Biology=== | ===Biology=== | ||
====SynNotch receptors function well in Lim WA et al in 2016==== | ====SynNotch receptors function well in Lim WA et al in 2016==== | ||
Revision as of 14:06, 9 October 2018
LaG17-mN1c-tTAA
This part is one of our SynNotch receptors[1]. LaG17 (Part:BBa_K2549004) is used as the extracellular sensor module to receive the signal input from GFP. mN1c (Part:BBa_K2549006) is served as the transmembrane core domain of SynNotch, which is evident to have a low basal expression and a high activation efficiency. tTAA (Part:BBa_K2446057) is an improved tetracycline-controlled transcription activator, which is cleaved after SynNotch activation and drives the expression of the amplifier. Besides, a CD8α signal peptide (Part:BBa_K2549044) and a Myc-tag (Part:BBa_K823036) are added to the N terminal of LaG17(Part:BBa_K2549004) for membrane targeting and easy determination of surface expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 87
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2154
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 127
Illegal SapI.rc site found at 1069
References
- ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012