Difference between revisions of "Part:BBa K2610011"
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| − | The SecA gene, coding for a protein translocase subunit, plays a vital role in protein transport across the cell membrane. | + | The SecA gene, coding for a protein translocase subunit, plays a vital role in protein transport across the cell membrane.<span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pmc/articles/PMC211308/ (Schmidt et al, 1988)]</span> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 22:07, 15 October 2018
pSecA
The SecA gene, coding for a protein translocase subunit, plays a vital role in protein transport across the cell membrane.(Schmidt et al, 1988)
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the SecA promoter is highly activated by kanamycin
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]