Difference between revisions of "Part:BBa K2717013:Design"
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===Source=== | ===Source=== | ||
| − | VHb | + | We obtained the sequence expressing the VHb protein from part BBa_K1321200 by PCR, and obtained the TorA signal sequence by gene synthesis. We linked these two sequences to the linearized vector by infusion. We placed the TorA sequence upstream of the VHb sequence and initiated expression by a promoter. |
===References=== | ===References=== | ||
Revision as of 17:57, 17 October 2018
TorA signal-VHb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 540
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
VHb
Source
We obtained the sequence expressing the VHb protein from part BBa_K1321200 by PCR, and obtained the TorA signal sequence by gene synthesis. We linked these two sequences to the linearized vector by infusion. We placed the TorA sequence upstream of the VHb sequence and initiated expression by a promoter.