Difference between revisions of "Part:BBa K2533046"
Line 1: Line 1:
−
 
+
transport of lactic acid
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2533046 short</partinfo>
+
<partinfo>BBa_K2533045 short</partinfo>
  
−
transport of lactic acid
+
produce latic acid
 +
 
 +
<h1>'''Usage and biology'''</h1>
 +
dld refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. With the overexpression of dld, Shewanella could utilize D-lactate more efficiently, which brings more electricity being produced.
  
−
<!-- Add more about the biology of this part here
+
<h1>'''Characterization'''</h1>
−
===Usage and Biology===
+
This is one section for lactate utilization part.
 +
[[File:T--HUST-China--2018-tonglu-lldP.png ‎|400px|thumb|center|Figure1:RBS-lldP]]
  
−
<!-- -->
+
<h2>DNA Gel Electrophoretic</h2>
−
<span class='h3bb'>Sequence and Features</span>
+
To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.
−
<partinfo>BBa_K2533046 SequenceAndFeatures</partinfo>
+
[[File:T--HUST-China--2018-tonglu-mles.png|400px|thumb|center|Figure2:Verification of successful transformation of pSB1C3-RBS-mleS]]
 +
Our target genes are 1969bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.
  
 +
<h2>Electrogenesis</h2>
 +
By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.
 +
[[File:T--HUST-China--2018-expression of lactate.png ‎|400px|thumb|center|Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.]]
 +
It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2533046 parameters</partinfo>
+
<partinfo>BBa_K2533030 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 14:27, 17 October 2018

transport of lactic acid

RBS-mleS

produce latic acid

Usage and biology

dld refers to FAD-dependent D-lactate dehydrogenase which could catalyze D-lactate’s transformation into pyruvate. With the overexpression of dld, Shewanella could utilize D-lactate more efficiently, which brings more electricity being produced.

Characterization

This is one section for lactate utilization part.

File:T--HUST-China--2018-tonglu-lldP.png
Figure1:RBS-lldP

DNA Gel Electrophoretic

To make sure that we get the target gene, we did the DNA gel electrophoretic to separate different gene. And here is the result.

Figure2:Verification of successful transformation of pSB1C3-RBS-mleS

Our target genes are 1969bp, and as the marker is DS5000, we could be sure that the bright bands in this picture are our target genes.

Electrogenesis

By comparing the ability of producing electricity, we might find out whether dld could effectively help Shewanella to produce more electricity.

Figure3:shows that our modification is effective. Every gene circuits can help strains produce lactate, and mleS-lldP-ldhA is the most efficient one. Therefore, our construction of gene circuits achieve the goal to help strains produce lactate.

It could be demonstrated that targeted genes could be expressed in the engineered cells. More NADH has been produced by engineered bacteria, which helps to produce more electricty.