Difference between revisions of "Part:BBa K2549016"

Revision as of 12:47, 9 October 2018

LaG17-mN1c-tTAA

This part is one of our SynNotch receptors^[1]. LaG17 (Part:BBa_K2549004) is used as the extracellular sensor module to receive the signal input from GFP. mN1c (Part:BBa_K2549006) is served as the transmembrane core domain of SynNotch, which is evident to have a low basal expression and a high activation efficiency. tTAA (Part:BBa_K2446057) is an improved tetracycline-controlled transcription activator, which is cleaved after SynNotch activation and drives the expression of the amplifier. Besides, a CD8α signal peptide (Part:BBa_K2549044) and a Myc-tag (Part:BBa_K823036) are added to the N terminal of LaG17(Part:BBa_K2549004) for membrane targeting and easy determination of surface expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 87
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2154
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 127
Illegal SapI.rc site found at 1069

References

↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012

[1] Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012

[1]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2549016 short</partinfo>
-This part is one of our SynNotch receptors<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. LaG17 ([[Part:BBa_K2549004]]) is used as the extracellular sensor module to receive the signal input from GFP. mN1c ([[Part:BBa_K2549006]]) is served as the transmembrane core domain of SynNotch, which is evident to have a low basal expression and a high activation efficiency. tTAA ([[Part: BBa_K2446057]]) is an improved tetracycline-controlled transcription activator, which is cleaved after SynNotch activation and drives the expression of the amplifier. Besides, a CD8&alpha; signal peptide ([[Part: BBa_K2549044]]) and a Myc-tag ([[Part: BBa_K823036]]) are added to the N terminal of LaG17([[Part:BBa_K2549004]]) for membrane targeting and easy determination of surface expression.
+This part is one of our SynNotch receptors<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. LaG17 ([[Part:BBa_K2549004]]) is used as the extracellular sensor module to receive the signal input from GFP. mN1c ([[Part:BBa_K2549006]]) is served as the transmembrane core domain of SynNotch, which is evident to have a low basal expression and a high activation efficiency. tTAA ([[Part:BBa_K2446057]]) is an improved tetracycline-controlled transcription activator, which is cleaved after SynNotch activation and drives the expression of the amplifier. Besides, a CD8&alpha; signal peptide ([[Part:BBa_K2549044]]) and a Myc-tag ([[Part:BBa_K823036]]) are added to the N terminal of LaG17([[Part:BBa_K2549004]]) for membrane targeting and easy determination of surface expression.
 <!-- Add more about the biology of this part here