Difference between revisions of "Part:BBa K2690002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | Priers for Biobrick construction and PCR amplification. | ||
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| + | Forward Primer | ||
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| + | 5’ – GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT CAC AAC GCT TTA TAC CTA ACC– 3’ | ||
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| + | Reverse Primer | ||
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| + | 5’ – GT TTC TTC CTG CAG CGG CCG CTA CTA GTA TTA TTA ACG TAC GCT TTT TTT GAT GGC – 3’ | ||
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| + | Notes: forward G-C content = 25/55 = 45%; reverse G-C content = 25/56 = 46% | ||
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Revision as of 12:43, 9 October 2018
PvsB - Vibrio parahaemolyticus vibrioferrin synthesis gene B
This coding sequence is part of the biosynthetic gene cluster for the synthesis and transport of the siderophore vibrioferrin. It's specific function in this process to to code for an enzyme for amide bond formation between 2-ketoglutarate and L-alanine, or L-alanine and ethanolamine (Fujita et al., 2011).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 555
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 684
- 1000COMPATIBLE WITH RFC[1000]