Difference between revisions of "Part:BBa K2610002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br> | + | <p>iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) </p> <br> |
[[File:T--Leiden--heatmap.png|750px]] <br> | [[File:T--Leiden--heatmap.png|750px]] <br> | ||
<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | ||
| − | As can be observed in Figure 1 the | + | As can be observed in Figure 1 the IbpA promoter is only moderately activated by streptomycin and tetracycline. |
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Revision as of 12:28, 9 October 2018
pIbpA
Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the IbpA promoter is only moderately activated by streptomycin and tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]