Difference between revisions of "Part:BBa K2610002"
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===Usage and Biology===
 
===Usage and Biology===
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<p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. <br> iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br>
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<p>Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli.
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iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1) </p> <br>
  
 
[[File:T--Leiden--heatmap.png|600px]] <br>
 
[[File:T--Leiden--heatmap.png|600px]] <br>
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<span style="font-size:0.9em"><b>Figure 1.</b> Heatmap</span>  
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<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span>  
  
 
As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.
 
As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.

Revision as of 12:19, 9 October 2018


pIbpA

Usage and Biology

Small heat shock proteins IbpA and IbpB are involved in heat shock response and oxidative stress. The promoter of IbpA has previously been defined as a possible stress probe in the heat shock response of E.coli. iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated against a repertoire of known antibiotics (Figure 1)


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the ibpA promoter is only moderately activated by streptomycin and tetracycline.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]