Difference between revisions of "Part:BBa K2703010"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2703010 short</partinfo>
 
<partinfo>BBa_K2703010 short</partinfo>
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2703010 SequenceAndFeatures</partinfo>
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=='''Introduction'''==
 +
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<h3> 1- Biological background </h3>
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 +
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<h3> 2- Usage in iGEM projects </h3>
 
The GAG pol part contain all the activities necessary for the transposition (RNA binding protein for gag, reverse transcription, intregrase and RNAseH for pol)
 
The GAG pol part contain all the activities necessary for the transposition (RNA binding protein for gag, reverse transcription, intregrase and RNAseH for pol)
 +
 +
=='''Sequencing'''==
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=='''Characterization'''==
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=='''References'''==
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<ol>
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<li> Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
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Pierre Crozet, Francisco J. Navarro, Felix Willmund, Payam Mehrshahi, Kamil Bakowski, Kyle J. Lauersen, Maria-Esther Pérez-Pérez, Pascaline Auroy, Aleix Gorchs Rovira, Susana Sauret-Gueto, Justus Niemeyer, Benjamin Spaniol, Jasmine Theis, Raphael Trösch, Lisa-Desiree Westrich, Konstantinos Vavitsas, Thomas Baier, Wolfgang Hübner, Felix de Carpentier, Mathieu Cassarini, Antoine Danon, Julien Henri, Christophe H. Marchand, Marcello de Mia, Kevin Sarkissian, David C. Baulcombe, Gilles Peltier, José-Luis Crespo, Olaf Kruse, Poul-Erik Jensen, Michael Schroda, Alison G. Smith, and Stéphane D. Lemaire
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ACS Synthetic Biology 2018 7 (9), 2074-2086 </li>
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DOI: 10.1021/acssynbio.8b00251 <br>
 +
<li> Weber, E., Engler, C., Gruetzner, R., Werner, S. &amp; Marillonnet, S. A modular cloning system for
 +
standardized assembly of multigene constructs. PLoS One 6, (2011). </li>
 +
</ol>
  
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2703010 SequenceAndFeatures</partinfo>
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Latest revision as of 16:56, 9 October 2018

GAG-POL

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

1- Biological background


2- Usage in iGEM projects

The GAG pol part contain all the activities necessary for the transposition (RNA binding protein for gag, reverse transcription, intregrase and RNAseH for pol)

Sequencing

Characterization

References

  1. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii Pierre Crozet, Francisco J. Navarro, Felix Willmund, Payam Mehrshahi, Kamil Bakowski, Kyle J. Lauersen, Maria-Esther Pérez-Pérez, Pascaline Auroy, Aleix Gorchs Rovira, Susana Sauret-Gueto, Justus Niemeyer, Benjamin Spaniol, Jasmine Theis, Raphael Trösch, Lisa-Desiree Westrich, Konstantinos Vavitsas, Thomas Baier, Wolfgang Hübner, Felix de Carpentier, Mathieu Cassarini, Antoine Danon, Julien Henri, Christophe H. Marchand, Marcello de Mia, Kevin Sarkissian, David C. Baulcombe, Gilles Peltier, José-Luis Crespo, Olaf Kruse, Poul-Erik Jensen, Michael Schroda, Alison G. Smith, and Stéphane D. Lemaire ACS Synthetic Biology 2018 7 (9), 2074-2086
  2. DOI: 10.1021/acssynbio.8b00251

  3. Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A modular cloning system for standardized assembly of multigene constructs. PLoS One 6, (2011).