Difference between revisions of "Part:BBa K2703009"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2703009 short</partinfo>
 
<partinfo>BBa_K2703009 short</partinfo>
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2703009 SequenceAndFeatures</partinfo>
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=='''Introduction'''==
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<h3> 1- Biological background </h3>
 
pSAD is a strong constitutive promotor in Chlamydomonas. it regulates a gene which encodes an abundant chloroplast protein located on the stromal side of the Photosystem I complex. This parts is the 5'UTR of the promotor of pSAD.  
 
pSAD is a strong constitutive promotor in Chlamydomonas. it regulates a gene which encodes an abundant chloroplast protein located on the stromal side of the Photosystem I complex. This parts is the 5'UTR of the promotor of pSAD.  
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<h3> 2- Usage in iGEM projects </h3>
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=='''Sequencing'''==
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=='''Characterization'''==
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=='''References'''==
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<ol>
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<li> Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
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Pierre Crozet, Francisco J. Navarro, Felix Willmund, Payam Mehrshahi, Kamil Bakowski, Kyle J. Lauersen, Maria-Esther Pérez-Pérez, Pascaline Auroy, Aleix Gorchs Rovira, Susana Sauret-Gueto, Justus Niemeyer, Benjamin Spaniol, Jasmine Theis, Raphael Trösch, Lisa-Desiree Westrich, Konstantinos Vavitsas, Thomas Baier, Wolfgang Hübner, Felix de Carpentier, Mathieu Cassarini, Antoine Danon, Julien Henri, Christophe H. Marchand, Marcello de Mia, Kevin Sarkissian, David C. Baulcombe, Gilles Peltier, José-Luis Crespo, Olaf Kruse, Poul-Erik Jensen, Michael Schroda, Alison G. Smith, and Stéphane D. Lemaire
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ACS Synthetic Biology 2018 7 (9), 2074-2086 </li>
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DOI: 10.1021/acssynbio.8b00251 <br>
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<li> Weber, E., Engler, C., Gruetzner, R., Werner, S. &amp; Marillonnet, S. A modular cloning system for
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standardized assembly of multigene constructs. PLoS One 6, (2011). </li>
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</ol>
  
 
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<span class='h3bb'>Sequence and Features</span>
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Latest revision as of 16:50, 9 October 2018

5'UTR pSAD

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

1- Biological background

pSAD is a strong constitutive promotor in Chlamydomonas. it regulates a gene which encodes an abundant chloroplast protein located on the stromal side of the Photosystem I complex. This parts is the 5'UTR of the promotor of pSAD.

2- Usage in iGEM projects

Sequencing

Characterization

References

  1. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii Pierre Crozet, Francisco J. Navarro, Felix Willmund, Payam Mehrshahi, Kamil Bakowski, Kyle J. Lauersen, Maria-Esther Pérez-Pérez, Pascaline Auroy, Aleix Gorchs Rovira, Susana Sauret-Gueto, Justus Niemeyer, Benjamin Spaniol, Jasmine Theis, Raphael Trösch, Lisa-Desiree Westrich, Konstantinos Vavitsas, Thomas Baier, Wolfgang Hübner, Felix de Carpentier, Mathieu Cassarini, Antoine Danon, Julien Henri, Christophe H. Marchand, Marcello de Mia, Kevin Sarkissian, David C. Baulcombe, Gilles Peltier, José-Luis Crespo, Olaf Kruse, Poul-Erik Jensen, Michael Schroda, Alison G. Smith, and Stéphane D. Lemaire ACS Synthetic Biology 2018 7 (9), 2074-2086

    2. DOI: 10.1021/acssynbio.8b00251

  2. Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A modular cloning system for standardized assembly of multigene constructs. PLoS One 6, (2011).