Difference between revisions of "Part:BBa K2847000:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
Standard design
+
Standard design  
  
  
 +
If you want to have this part synthesized by IDT, order the following sequence:
 +
 +
<pre>
 +
gRNA cloning cassette
 +
gaattcgcggccgcttctagagCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGA
 +
TTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATCGCGCAGTTTTTAGGGTGGTTTG
 +
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCC
 +
TCGTAGCCATCGAGATAAACCGCAGAAATCGTGTCAGCCAGCAGCCTGGATTTTTTCGTtactagtagcggccgctgcag</pre>
 +
 +
 +
PCR amplify it using the following primers:
 +
 +
C_BioBrick_Prefix-F: TATGAATTCGCGGCCGCTTCTAG
 +
 +
C_BioBrick_Suffix-R: TATCTGCAGCGGCCGCTACTAGTA
 +
                         
 +
 +
 +
and then clone it into a pSB vector of choice.
  
 
===Source===
 
===Source===

Latest revision as of 20:21, 17 October 2018


Cer sequence from plasmid ColE1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard design


If you want to have this part synthesized by IDT, order the following sequence: 

gRNA cloning cassette
gaattcgcggccgcttctagagCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGA
TTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATCGCGCAGTTTTTAGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCC
TCGTAGCCATCGAGATAAACCGCAGAAATCGTGTCAGCCAGCAGCCTGGATTTTTTCGTtactagtagcggccgctgcag


PCR amplify it using the following primers:

C_BioBrick_Prefix-F: TATGAATTCGCGGCCGCTTCTAG

C_BioBrick_Suffix-R: TATCTGCAGCGGCCGCTACTAGTA


and then clone it into a pSB vector of choice.

Source

This part was obtained as gBlock fragment by IDT

References