Difference between revisions of "Part:BBa K2539250"

Revision as of 03:52, 10 October 2018

ALDH2*2 Basic Part

This gene codes for the mutant form of human mitochondrial acetaldehyde dehydrogenase, ALDH2*2, which metabolizes acetaldehyde at a slower rate compared to wild type ALDH2*2 (Chen et al., 2014).

The ALDH2*2 sequence was based off of ALDH2*1 (NCBI: NM_001204889.1), but modified to remove an internal PstI site. Compared to wild type ALDH2*1, the mutant ALDH2*2 contains a single G to A point mutation, which leads to a change in codon 487, where GAA (coding for glutamic acid) is mutated to AAA (coding for lysine) (Enomoto et al., 1991). The part was confirmed by PCR using the primers VF2 and VR, as well as sequencing by Tri-I Biotech.

PCR Check Results

PCR Check for BBa_K2539250 using the forward and reverse primers VF2 and VR. The expected PCR size of BBa_K2539250 (ALDH2*2 basic part) is around 1800 bp. Bands with the correct size are boxed in yellow.

References

Chen CH, Ferreira JCB, Gross ER, Mochly-Rosen D. (2014). Targeting Aldehyde Dehydrogenase 2: New Therapeutic Opportunities. Physiol Rev. 94(1):1-34.

Enomoto N, Takada A, Date T. (1991). Genotyping of the aldehyde dehydrogenase 2 (ALDH2) gene using the polymerase chain reaction: evidence for single point mutation in the ALDH2 gene of ALDH2-deficiency. Gastroenterol Jpn. 26(4): 440–447.

Sequence and Features