Difference between revisions of "Part:BBa K2570019"
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The Tet-Off system which turns expression of its controlling genes OFF by the addition of tetracycline and its derivative is often used for this purpose and has been applied to several organisms. | The Tet-Off system which turns expression of its controlling genes OFF by the addition of tetracycline and its derivative is often used for this purpose and has been applied to several organisms. | ||
− | The figure shows a strategy for metabolic state switching module using a synthetic metabolic regulator. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator circuit. AraC repressor inhibits transcription from the PBAD promoter. | + | The figure shows a strategy for metabolic state switching module using a synthetic metabolic regulator. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator circuit. AraC repressor inhibits transcription from the PBAD promoter. When transcription by the PBAD promoter is induced without arabinose addition, expression of tetR and GFP is promoted and the expression of mCherry under the PLtetO1 promoter is inhibited by the TetR repressor. |
[[File:FileT--FJNU-China--TET-OFF_1.png|600px|thumb|center|Fig. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator.]] | [[File:FileT--FJNU-China--TET-OFF_1.png|600px|thumb|center|Fig. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator.]] |
Revision as of 13:51, 9 October 2018
tetR+GFP+mazF+PLtetO1+RFP
The Tet-Off system which turns expression of its controlling genes OFF by the addition of tetracycline and its derivative is often used for this purpose and has been applied to several organisms.
The figure shows a strategy for metabolic state switching module using a synthetic metabolic regulator. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator circuit. AraC repressor inhibits transcription from the PBAD promoter. When transcription by the PBAD promoter is induced without arabinose addition, expression of tetR and GFP is promoted and the expression of mCherry under the PLtetO1 promoter is inhibited by the TetR repressor.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2590
Illegal AgeI site found at 2702 - 1000COMPATIBLE WITH RFC[1000]