Difference between revisions of "Part:BBa K2889003:Experience"
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We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI | We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI | ||
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+ | We selected the positive clones by PCR and sequencing | ||
===User Reviews=== | ===User Reviews=== |
Revision as of 04:43, 9 October 2018
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K2889003
First we amplified miR-21-sponge-4s using primers. After that, we purified the PCR products by PCR Purification Kit and digested them with restriction enzymes EcoR I and Pst I
We digested the PSB1C3 vectors with restriction enzymes EcoRI and PstI
We selected the positive clones by PCR and sequencing
User Reviews
UNIQ300b172a4e771294-partinfo-00000000-QINU UNIQ300b172a4e771294-partinfo-00000001-QINU