Difference between revisions of "Part:BBa K2683001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Although originally codon optimized for E.coli, sequencing issues lead us to working with genomic DNA. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Revision as of 22:36, 8 October 2018
Arc Protein Codon optimized for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 531
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Although originally codon optimized for E.coli, sequencing issues lead us to working with genomic DNA.
Source
The original sequence is derived from the Arc protein found in mouse neural cells. The DNA was amplified from the mouse genome and made biobrick compatable by using primer extension PCR and site directed mutagenesis.