Difference between revisions of "Part:BBa K2570000"
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Construction and optimization of the 2-PE biosynthetic pathway in  ''E.coli''.
 
Construction and optimization of the 2-PE biosynthetic pathway in  ''E.coli''.
  
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A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1), phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .
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A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in ''E. coli'' by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1)https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570000 BBa_K2570002], phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .
  
  

Revision as of 04:27, 10 October 2018


PAR

To optimize the engineered 2-PE biosynthetic pathway, the Par (BAG13450.2) the phenyla- cetaldehyde reductase from the rose (Rosa sp.)

Construction and optimization of the 2-PE biosynthetic pathway in E.coli.

A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1)https://parts.igem.org/wiki/index.php?title=Part:BBa_K2570000 BBa_K2570002], phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenyla- cetaldehyde reductase from the rose (Par , BAG13450.2 ) under the control of the constitutive promoter (BBa_J23100). The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 247
    Illegal NotI site found at 610
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]