Difference between revisions of "Part:BBa K2665013"
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AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles and that original comes from Arabidopsis thaliana.
 
AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles and that original comes from Arabidopsis thaliana.
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We assembled '''[https://parts.igem.org/Part:BBa_K2225000 BBa_K2225000]''' with '''[https://parts.igem.org/Part:BBa_K124002 TDpromoter(BBa_K124002)]''' and '''[https://parts.igem.org/Part:BBa_K2665010 CYC1 terminator(BBa_K2665010)]'''.  
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We assembled '''[https://parts.igem.org/Part:BBa_K2225000 BBa_K2225000]''' with TDpromoter and CYC1 terminator to express the protein, and we added His-tag in case of Western blotting.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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===Usage and Biology===
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==Usage and Biology==
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This part sequence can be used directly because it contains promoter and terminator, but promoter is TDH3 promoter so it can't express in <i>E. coli</i>. AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles, so we used this part in order to increase the amount of Na+ uptake of yeast.
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==Characterization==
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<!--續さん　ここにお願いします-->
  
 
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Revision as of 01:52, 9 October 2018


TDH3-AtNHXS1-6xHis-CYC

AtNHXS1 is a Na+/H+ antiporter which is located in the membrane of vacuoles and that original comes from Arabidopsis thaliana. We assembled BBa_K2225000 with TDpromoter and CYC1 terminator to express the protein, and we added His-tag in case of Western blotting.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]