Difference between revisions of "Part:BBa K2833009"
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<h1>Microscopy result</h1> | <h1>Microscopy result</h1> | ||
| − | We observed the fluorescence under the super-resolution microscope. As figure 1 shows, The intracellular expression of sfGFP displayed the rode-shape of E.coli, while the signal of surface displayed sfGFP showed the dotted pattern, which suggested that the sfGFP were gathered and distributed at the surface of <I>E.coli.</I>. | + | We observed the fluorescence under the super-resolution microscope. As figure 1 shows, The intracellular expression of sfGFP displayed the rode-shape of ii>E.coli</i>, while the signal of surface displayed sfGFP showed the dotted pattern, which suggested that the sfGFP were gathered and distributed at the surface of <I>E.coli.</I>. |
[[File:T--BJRS China--009.jpg|thumbnail|center|500px|<b>Fig.1 super-resolution microscopy of INP-sfGFP</b> Left: intracellular expression of GFP; Right: surface display of GFP via INP]]<br><br> | [[File:T--BJRS China--009.jpg|thumbnail|center|500px|<b>Fig.1 super-resolution microscopy of INP-sfGFP</b> Left: intracellular expression of GFP; Right: surface display of GFP via INP]]<br><br> | ||
Revision as of 12:46, 8 October 2018
J23104+B0032+INP+sfGFP
Ice nucleation protein(INP)is a secretory outer membrane protein from Pseudomomas syringae P. flurorescens and several other Gram—negative bacteria.In the bacterial surface display technology，INP is widely used as a functional carrier protein.
Design
We got the INP from BBa_K523013 constructed by iGEM11_Edinburgh. To test the surface display efficiency of INP, we added sfGFP to the downstream of INP, and expressed the fusion protein under the control of J23104 and B0032.
Microscopy result
We observed the fluorescence under the super-resolution microscope. As figure 1 shows, The intracellular expression of sfGFP displayed the rode-shape of ii>E.coli</i>, while the signal of surface displayed sfGFP showed the dotted pattern, which suggested that the sfGFP were gathered and distributed at the surface of E.coli..
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 467
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1050