Difference between revisions of "Part:BBa K2541101:Design"

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===Design Notes===
 
===Design Notes===
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When designing the sequence, you should consider its pairing with other bases, which may affect its free energy.
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The secondary structure is important to the function of an RNA thermosensor. We designed the thermosensor according to the stem length,loop size and base bulge.
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===Source===
 
===Source===

Revision as of 05:22, 8 October 2018


Heat-repressible RNA-based thermosensor-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 31


Design Notes

The secondary structure is important to the function of an RNA thermosensor. We designed the thermosensor according to the stem length,loop size and base bulge.

Source

This sequence is not derived from any organism. We designed this sequence on our own and synthesized this sequence from a synthesis company.

References

Hoynes-O'Connor A, Hinman K, Kirchner L, et al. De novo design of heat-repressible RNA thermosensors in E. coli[J]. Nucleic Acids Research, 2015, 43(12):6166-6179.