Difference between revisions of "Part:BBa K2683003"

Latest revision as of 03:16, 12 October 2018

MS2 Protein with Polyarginine Peptide

The MS2 protein is a capsid protein originally derived from the MS2 bacteriophage and has been shown to deliver macromolecules in application[1]. A surface modification was added, whereby a polyarginine peptide sequence of 15 Arginines is added to the C-terminus with a thrombin cleavage site in the linker [2]. Polyarginine tags are known to act as a non-specific Cell penetrating peptide[2]. This part was codon optimized for use in E.coli .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 63
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2683003 short</partinfo>
-The MS2 protein is a capsid protein originally derived from the MS2 bacteriphage. A surface modification was added whereby a polyarginine peptide sequence of 15 Arginines with a thrombin cleavage site in the linker. Polyarginine tags are known to act as a non-specific Cell penetrating peptide [Schmidt et al., 2010]. This part was codon optimized for use in E.coli.
+The MS2 protein is a capsid protein originally derived from the MS2 bacteriophage and has been shown to deliver macromolecules in application[1]. A surface modification was added, whereby a polyarginine peptide sequence of 15 Arginines is added to the C-terminus with a thrombin cleavage site in the linker [2]. Polyarginine tags are known to act as a non-specific Cell penetrating peptide[2]. This part was codon optimized for use in <i>E.coli </i>.
 <!-- Add more about the biology of this part here