Difference between revisions of "Part:BBa K2740015"
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2740015 parameters</partinfo>
 
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<h2>Parameter of Protein </h2>
 
<h2>Parameter of Protein </h2>
 
<p align="left">Number  of amino acids: 509</p>
 
<p align="left">Number  of amino acids: 509</p>
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<p >[[File:T--Nanjing-China--nif K-structure.png|800px|thumb|center]]</p>
 
<p >[[File:T--Nanjing-China--nif K-structure.png|800px|thumb|center]]</p>
 
<h2>Confirmation of Expression of nifK</h2>
 
<h2>Confirmation of Expression of nifK</h2>
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<p align="left">To  verify the expression of nitrogenase gene, we conducted Real-time Quantitative PCR(QPCR) to detect the transcription level of <em>nif</em> gene cluster in engineered <em>E.  coli</em>, using 16S DNA as an internal reference. The result provided the  relative expression level of each <em>nif</em>K  in our constructed <em>E. coli </em>strain. </p>
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<p>We test expression profiles of each  structure gene in the nif cluster that overexpressed in EJNC by conducting  Real-time Quantitative PCR(qPCR). Relative expression compared to the housekeeping gene 16S rRNA is shown.</p>
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[[File:T--Nanjing-China--nifK-1.png|800px|thumb|center]]<br/>
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[[File:T--Nanjing-China--nifK.jpg|800px|thumb|center]]
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[[File:T--Nanjing-China--nifK-2.png|800px|thumb|center]]
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<p>qRT-PCR analysis demonstrates that all the component genes of the nif cluster are significantly over expressed in EJNC whereas the transcription of these genes are no detected (N.D.) in nondiazotrophic E.coli JM109. Based on these analysis, we know nifK has a relatively low expression level.</p>
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<p>From the results of qPCR, we know the nifX gene in engineered <em>E. coli relatively fractionally expressed.</em></p>
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<div>
 
<div>
 
   <h2>Usage</h2>
 
   <h2>Usage</h2>

Revision as of 10:53, 15 October 2018


CR1 nifK

CR1 nifK encodes the subunit beta (NifK) of the molybdenum-iron protein. Together with NifD, they form a α2β2 heterotetramer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1106
    Illegal SapI.rc site found at 1489