Difference between revisions of "Part:BBa K2598030"
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<b>Figure 2</b> shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well. | <b>Figure 2</b> shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well. | ||
<div>[[File:T—UCAS-China—abc222.png|1000px|thumb|center|<b>Figure 2:</b>Relationship between fluorescence intensity and excitation wavelength]]</div> | <div>[[File:T—UCAS-China—abc222.png|1000px|thumb|center|<b>Figure 2:</b>Relationship between fluorescence intensity and excitation wavelength]]</div> | ||
| + | ===Characterization=== | ||
| + | <b>Figure 3</b> shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows. When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity. | ||
| + | <div>[[File:T—UCAS-China—FP SOLIDE.png|1000px|thumb|center|<b>Figure 3:</b> Colors we got from the solid medium, in which E. coli producing fluorescent protein grows, exposed under light]]</div> | ||
Revision as of 06:49, 9 October 2018
K1F-inducible promoter pK1F
This is a promoter that caan be induced by T7 RNAP sigma fragment K1F.When combined with T7 RNAP core fragment, K1F can direct the polymerase to this specific promoter, triggering expression of downstream genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
Figure 1 shows the relationship between the wavelength of light exposed on liquid medium and the intensity of BFP, GFP and RFP E. coli expressed from left figure to right figure respectively. We got the data through flow cytometer and analyzed it to get the figure. The y-axis is the number of cells, and the x-axis is fluorescence intensity. And every color is E. coli that grows for 8 hours under the light of the corresponding wavelength. We can see E. coli has the highest blue fluorescence expression under blue light from the left graph. And We can also see E. coli has the highest green and red fluorescence expression under green light and right light from the middle and right graph respectively. So this figure proves that our system and our parts can work well.
Characterization
Figure 2 shows the relationship between fluorescence intensity and excitation wavelength. The x-axis is wavelength of 10h illumination. The solid medium gradually emerged and the y-axis is RGB figure of fluorescence in illuminated solid medium. This curve illustrates how our system responses to different excitation wavelength, which perfectly meets our expectation. So this figure proves that our system and our parts can work well.
Characterization
Figure 3 shows colors we got from the solid medium exposed under light, in which E. coli producing fluorescent protein grows. When E .coli producing fluorescent protein are exposed under uniform light of single wavelength, the solid medium gradually emerged corresponding colors. And using color picker, we get many pure colors with predominant continuity.
