Difference between revisions of "Part:BBa K2653000"
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Trim21 is a type of E3 ubiquitin ligase that has been discovered in many eukaryotic cells.  
 
Trim21 is a type of E3 ubiquitin ligase that has been discovered in many eukaryotic cells.  
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The Tripartite motif-containing protein 21(Trim21) belongs to the E3 ubiquitin ligase family, which is encoded by the TRIM21 gene.
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2653000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2653000 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
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===Source===
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The sequence of Trim21 is from the NCBI database.
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<partinfo>BBa_K2653000 parameters</partinfo>
 
<partinfo>BBa_K2653000 parameters</partinfo>
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===Design Notes===
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There is 2 domains for PstI with the sequence of our Trim21, so we carried out point mutation to avoid this.
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==通常==
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There are 2 domains for PstI with the sequence of our Trim21, so we carried out point mutation to avoid this.
  
  

Revision as of 17:16, 17 October 2018


trim21

Trim21 is a type of E3 ubiquitin ligase that has been discovered in many eukaryotic cells.

Usage and Biology

The Tripartite motif-containing protein 21(Trim21) belongs to the E3 ubiquitin ligase family, which is encoded by the TRIM21 gene. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 873
    Illegal BamHI site found at 1411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1251


Source

The sequence of Trim21 is from the NCBI database.


通常

There are 2 domains for PstI with the sequence of our Trim21, so we carried out point mutation to avoid this.


Source

Its sequence come from articles.

References