Difference between revisions of "Part:BBa K2609026"

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===Usage and Biology===
 
===Usage and Biology===
 
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<h2> Biology </h2>
 
<h2> Biology </h2>
 
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     We used the this Lambda RED recombination system with a T4 Endolysin gene (<a href="https://parts.igem.org/Part:BBa_K2609017">BBa_K2609017</a>) to recombine Bacteriophage T4 and create a lysis deficient phage. This lysis deficient phage triggers its host to produce a monocyte chemoattractant <i>mcp-1</i> (<a href="https://parts.igem.org/Part:BBa_K2609000">BBa_K2609000</a>).
 
     We used the this Lambda RED recombination system with a T4 Endolysin gene (<a href="https://parts.igem.org/Part:BBa_K2609017">BBa_K2609017</a>) to recombine Bacteriophage T4 and create a lysis deficient phage. This lysis deficient phage triggers its host to produce a monocyte chemoattractant <i>mcp-1</i> (<a href="https://parts.igem.org/Part:BBa_K2609000">BBa_K2609000</a>).
 
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Revision as of 21:38, 7 October 2018


Lambda Red Recombinases under T7 expression system

This part generates the three Lambda ReD recombinases - exo, gam, beta - when expressed in a T7 expression strain like E. coli BL21(DE3).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1943
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1460
  • 1000
    COMPATIBLE WITH RFC[1000]