Difference between revisions of "Part:BBa K2547000"

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This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.
 
This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.
  <div style="width:100%" align="center">https://static.igem.org/mediawiki/parts/8/8c/T--AHUT_China--_sixiao1454.jpg</div>
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:09, 12 October 2018


Carbonic anhydrase 2


This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]