Difference between revisions of "Part:BBa K2675014"

Latest revision as of 21:05, 15 October 2018

Synthetic RBS designed for Tat-SAIRGA

This part is a synthetic RBS designed for Tat-SAIRGA (BBa_K2675004) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of Tat-SAIRGA (BBa_K2675004) under the control of the BBa_J23100 promoter in the composite part BBa_K2675044.

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	10020,84
dG_total (kcal/mol)	:	-4,65
dG_mRNA_rRNA (kcal/mol)	:	-3,12
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-1,49
Accuracy (warnings)	:	OK

REFERENCES

[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.

[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 18: / Line 18: @@
   Accuracy (warnings)	:	OK
+<h2 style="font-weight:800;">REFERENCES</h2>
+<p style="font-size:15px;" class="bibliographie">[1] Espah Borujeni A, Channarasappa AS, Salis HM. Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites. Nucleic Acids Res (2014) 42, 2646-2659.</p>
+<p style="font-size:15px;" class="bibliographie">[2] Salis HM, Mirsky EA, Voigt CA. Automated design of synthetic ribosome binding sites to control protein expression. Nat Biotechnol (2009) 27, 946-50.</p>
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 <span class='h3bb'>Sequence and Features</span>