Difference between revisions of "Part:BBa K2547000"

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This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.
 
This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.
  <div align="center"><img src="https://static.igem.org/mediawiki/parts/c/c9/T--AHUT_China--_shengwu1235.jpg" width="300" height="452" alt=""/></div>
+
  <div align="center"><img src="https://static.igem.org/mediawiki/parts/c/c9/T--AHUT_China--_shengwu1235.jpg" width="100" height="252" alt=""/></div>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:21, 7 October 2018


Carbonic anhydrase 2


This part is the coding sequence (CDS) of human carbonic anhydrase (CA-II), because Carbonic Anhydrase II (CA2) is a metalloenzyme that can efficiently catalyze the formation of HCO3- from CO2 and H2O. This reaction is highly efficient with H+. The reaction rate is extremely fast, and the histidine tag (His-Tag) is linked to the coding sequence (CDS) to facilitate the purification of carbonic anhydrase protein.

<img src="T--AHUT_China--_shengwu1235.jpg" width="100" height="252" alt=""/>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]