Difference between revisions of "Part:BBa K2686002"
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<partinfo>BBa_K2686002 short</partinfo> | <partinfo>BBa_K2686002 short</partinfo> | ||
− | This is a BioBrick containing the sequence for Thermotoga maritima encapsulin, a bacterial protein nanocompartment which self assembles to form a 60-mer. The part can be used to deliver cargo, both on the outer surface of the nanoparticle by fusing a peptide in between the 139/140 Amino Acids as well as the protein's C terminus | + | This is a BioBrick containing the sequence for Thermotoga maritima encapsulin, a bacterial protein nanocompartment which self assembles to form a 60-mer. The part can be used to deliver cargo, both on the outer surface of the nanoparticle by fusing a peptide in between the 139/140 Amino Acids as well as the protein's C terminus. Cargo proteins can also be loaded inside the nano-cage using a tag binding to Encapsulin's interior surface. The protein is modified with an additonal amino acid sequence (GGGGGGHHHHHHGGGGG) between positions 43/44 granting it better stability and high heat resistance [1]. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2686002 parameters</partinfo> | <partinfo>BBa_K2686002 parameters</partinfo> | ||
− | <!-- --> | + | <!-- References --> |
+ | [1] Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform | ||
+ | Hyojin Moon, Jisu Lee, Junseon Min, and Sebyung Kang | ||
+ | Biomacromolecules 2014 15 (10), 3794-3801 | ||
+ | DOI: 10.1021/bm501066m |
Revision as of 14:07, 8 October 2018
Encapsulin protein with HexaHistidine insert
This is a BioBrick containing the sequence for Thermotoga maritima encapsulin, a bacterial protein nanocompartment which self assembles to form a 60-mer. The part can be used to deliver cargo, both on the outer surface of the nanoparticle by fusing a peptide in between the 139/140 Amino Acids as well as the protein's C terminus. Cargo proteins can also be loaded inside the nano-cage using a tag binding to Encapsulin's interior surface. The protein is modified with an additonal amino acid sequence (GGGGGGHHHHHHGGGGG) between positions 43/44 granting it better stability and high heat resistance [1].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 77
Illegal BglII site found at 492 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 426
Illegal SapI.rc site found at 457
[1] Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform
Hyojin Moon, Jisu Lee, Junseon Min, and Sebyung Kang
Biomacromolecules 2014 15 (10), 3794-3801
DOI: 10.1021/bm501066m