Difference between revisions of "Part:BBa K2623007:Experience"
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SAHS is an exogenous protein with a signal peptide in front of it that allows the protein to be secreted outside the membrane. But our site organism is E. coli, because there is a cell wall, so the protein can not be secreted out of the membrane. Therefore, we can hardly see our target protein in the supernatant. Therefore, we can only obtain the protein we need from the cell pellet. After that, we connected our SAHS protein to the pET-28a plasmid, induced expression, and obtained a large amount of our target protein by ultrasonic disruption of the bacterial cell pellet.<br> | SAHS is an exogenous protein with a signal peptide in front of it that allows the protein to be secreted outside the membrane. But our site organism is E. coli, because there is a cell wall, so the protein can not be secreted out of the membrane. Therefore, we can hardly see our target protein in the supernatant. Therefore, we can only obtain the protein we need from the cell pellet. After that, we connected our SAHS protein to the pET-28a plasmid, induced expression, and obtained a large amount of our target protein by ultrasonic disruption of the bacterial cell pellet.<br> | ||
| − | More information about our project can be found on our results page | + | More information about our project can be found on our results page http://2018.igem.org/Team:XMU-China/Results |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 10:00, 7 October 2018
Applications of BBa_K2623007
Identification
We performed a small amount of protein expression by SDS-PAGE.
SAHS is an exogenous protein with a signal peptide in front of it that allows the protein to be secreted outside the membrane. But our site organism is E. coli, because there is a cell wall, so the protein can not be secreted out of the membrane. Therefore, we can hardly see our target protein in the supernatant. Therefore, we can only obtain the protein we need from the cell pellet. After that, we connected our SAHS protein to the pET-28a plasmid, induced expression, and obtained a large amount of our target protein by ultrasonic disruption of the bacterial cell pellet.
More information about our project can be found on our results page http://2018.igem.org/Team:XMU-China/Results
User Reviews
UNIQ9ed5b1acdf320b2c-partinfo-00000000-QINU UNIQ9ed5b1acdf320b2c-partinfo-00000001-QINU