Difference between revisions of "Part:BBa K2665011"
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<partinfo>BBa_K2665011 short</partinfo> | <partinfo>BBa_K2665011 short</partinfo> | ||
− | + | This part coded the mangrin functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast. | |
− | This part coded the functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast. | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Mangrin is derived from mangrove and it is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. This part contains only the functional domain(71 amino acids). TDH3 promoter is a constitutive promoter, so this part can express mangrin functional domain constitutively under appropriate condition. | ||
+ | |||
+ | ==Characterization== | ||
For the confirmation of the mangrin function of mangrin, we spotted | For the confirmation of the mangrin function of mangrin, we spotted | ||
Revision as of 10:05, 7 October 2018
TDH3-Mangrin-6xHis-CYC
This part coded the mangrin functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 842
Illegal BamHI site found at 936 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910