Difference between revisions of "Part:BBa K2665011"
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===Usage and Biology===
 
===Usage and Biology===
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For the confirmation of the mangrin function of mangrin, we spotted
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<partinfo>BBa_K2665011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2665011 SequenceAndFeatures</partinfo>
  
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==Characterization==
 
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For the confirmation of the mangrin function of mangrin, we spotted
 
  
 
==Reference==
 
==Reference==

Revision as of 09:58, 7 October 2018


TDH3-Mangrin-6xHis-CYC

Mangrin is derived from mangrove and it is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells.
This part coded the functional domain(71 amino acids). This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. We used this part for improving halotorelance of yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 842
    Illegal BamHI site found at 936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910