Difference between revisions of "Part:BBa K2665011"
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==Characterization==
 
==Characterization==
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In order to demonstrate the function of the Rev protein and RRE, we fused an RRE [1] to U6 snRNA, which has a complex structure and is not transported by the TAP / p15 pathway. In Xenopus oocytes, we ascertained whether this RNA was exported from the nucleus in a Rev-dependent manner.
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For the confirmation of the mangrin function of mangrin, we spotted
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==Reference==
 
==Reference==

Revision as of 09:52, 7 October 2018


TDH3-Mangrin-6xHis-CYC

This part coded salt tolerance protein, Mangrin form mangrove. This part also contains TDH3 promoter, Histag, and CYC1 terminator in order to express constantly in yeasts. Mangrin is a shaperon like protein and enhances salt tolerance of Escherichia coli, Yeast, and Tobacco Cells. We used this part for improving halotorelance of yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 842
    Illegal BamHI site found at 936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

For the confirmation of the mangrin function of mangrin, we spotted

Reference

[1] A. Yamada, T. Saitoh, T. Mimura et al. (2002) Expression of Mangrove Allene Oxide Cyclase Enhances Salt Tolerance in Escherichia coli, Yeast, and Tobacco Cells, Plant and cell physiology 903-910