Difference between revisions of "Part:BBa K2888010:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
Latest revision as of 02:51, 10 October 2018
fused mlrA gene with histag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 770
Illegal AgeI site found at 305 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We add 6x HIS to the existed DNA sequence
Source
This mlrA gene is a part of the microcystin degradation system discovered in Sphingopyxis sp. C-1 by Shimuzu.