Difference between revisions of "Part:BBa K2549013"
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<partinfo>BBa_K2549013 short</partinfo>
 
<partinfo>BBa_K2549013 short</partinfo>
  
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This part is a mutant TEV (tobacco etch virus) protease. The 219 residue of the wild-type TEV protease is mutated from serine to valine to remove autoinactivation and perform a more efficient cleavage<ref>Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust RB, Tözsér J, Fox JD, ..., Copeland TD, Waugh DS. Protein Eng, 2001 Dec;14(12):993-1000  PMID: 11809930</ref>. TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. We used it to build our IMPLY logic gates. It can also be utilized by other iGEM teams to create their own engineered fusion proteins cleavage system.
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This part is a mutant TEV (tobacco etch virus) protease. The residue 219 of the wild-type TEV protease is mutated from serine to valine (S219V) to remove autoinactivation and perform a more efficient cleavage<ref>Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust RB, Tözsér J, Fox JD, ..., Copeland TD, Waugh DS. Protein Eng, 2001 Dec;14(12):993-1000  PMID: 11809930</ref>. TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. We used it to build our IMPLY logic gates. It can also be utilized by other iGEM teams to create their own engineered fusion proteins cleavage system.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 05:11, 7 October 2018


TEV protease

This part is a mutant TEV (tobacco etch virus) protease. The residue 219 of the wild-type TEV protease is mutated from serine to valine (S219V) to remove autoinactivation and perform a more efficient cleavage[1]. TEV protease is one of the best-characterized enzymes of the viral proteases which have more stringent sequence specificity. We used it to build our IMPLY logic gates. It can also be utilized by other iGEM teams to create their own engineered fusion proteins cleavage system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316

Usage and Biology

TBA



References

  1. ↑ Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Kapust RB, Tözsér J, Fox JD, ..., Copeland TD, Waugh DS. Protein Eng, 2001 Dec;14(12):993-1000 PMID: 11809930