Difference between revisions of "Part:BBa K2549003"
Line 3: Line 3:
 
<partinfo>BBa_K2549003 short</partinfo>
 
<partinfo>BBa_K2549003 short</partinfo>
  
−
Anti-GFP (LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170</ref>. It was used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. Two copies of LaG16 joined to form LaG2 ([[Part:BBa_K2549002]]) using a flexible glycine-rich peptide linker, which is a dimerized, ultra-high affinity antibody against GFP.
+
Anti-GFP (LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170</ref>. It was used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. Two copies of LaG16 joined to form LaG2 ([[Part:BBa_K2549002]]) using a flexible glycine-rich peptide linker, which is a dimerized, ultra-high affinity antibody against GFP. This nanobody recognizes our surEGFP ([[Part:BBa_K2446051]]) expressing cells.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 05:03, 7 October 2018


anti-GFP (LaG16)

Anti-GFP (LaG16) is a readily expressible recombinant nanobody which has a high affinity and high specificity against GFP[1]. It was used as the extracellular domain of the SynNotch, thus accomplishing the contact-dependent signal input against GFP. Two copies of LaG16 joined to form LaG2 (Part:BBa_K2549002) using a flexible glycine-rich peptide linker, which is a dimerized, ultra-high affinity antibody against GFP. This nanobody recognizes our surEGFP (Part:BBa_K2446051) expressing cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

TBA



References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170