Difference between revisions of "Part:BBa K2680067"

Revision as of 01:23, 18 October 2018

J23107 TPL + ptplAr B0034 mScarlet-I pdt#3b

Medium-weak 39C Temperature-Sensitive (ptplAr) mScarlet-I pdt#3b

Components: J23107 TPL + ptplAr B0034 mScarlet-I pdt#3b

Mechanics J23107, a medium-weak constitutive promoter, constitutively expresses TlpA39. Unlike the original TlpA, TlpA39 undergoes a conformational change at 39C and loses its ability to bind the downstream pTlpA. Therefore, TlpA39 halts its repression of weak promoter pTlpAr at temperatures above 39C, inducing the expression of the rapidly-maturing mScarlet-I. The mScarlet-I in this circuit has a pdt degradation tag, allowing for degradation by the mf-Lon protease. Specifically, this circuit contains pdt#3b, a medium-strong degradation tag from part series K2333401-K2333406 or BBa_K2333403.

As an IFFL In this circuit, heat activates a reporter, mScarlet-I. In a complete IFFL (incoherent feed forward loop), an input must activate both a reporter and its inhibitor. Therefore, this circuit requires mf-Lon (or a different inhibitor of mScarlet-I) to act as an IFFL.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 51
Illegal NheI site found at 74
Illegal NotI site found at 613
Illegal NotI site found at 1920
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 898
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1201
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2680067 short</partinfo>
-Undef
+Medium-weak 39C Temperature-Sensitive (ptplAr) mScarlet-I pdt#3b
+Components: J23107 TPL + ptplAr B0034 mScarlet-I pdt#3b
+Mechanics
+J23107, a medium-weak constitutive promoter, constitutively expresses TlpA39. Unlike the original TlpA, TlpA39 undergoes a conformational change at 39C and loses its ability to bind the downstream pTlpA. Therefore, TlpA39 halts its repression of weak promoter pTlpAr at temperatures above 39C, inducing the expression of the rapidly-maturing mScarlet-I. The mScarlet-I in this circuit has a pdt degradation tag, allowing for degradation by the mf-Lon protease. Specifically, this circuit contains pdt#3b, a medium-strong degradation tag from part series K2333401-K2333406 or <partinfo>BBa_K2333403</partinfo>.
+As an IFFL
+In this circuit, heat activates a reporter, mScarlet-I. In a complete IFFL (incoherent feed forward loop), an input must activate both a reporter and its inhibitor. Therefore, this circuit requires mf-Lon (or a different inhibitor of mScarlet-I) to act as an IFFL.
 <!-- Add more about the biology of this part here