Difference between revisions of "Part:BBa Q04401"
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This is a modified version of the tetR inverter <bbpart>Q04400</bbpart> with a single base switch in the RBS: | This is a modified version of the tetR inverter <bbpart>Q04400</bbpart> with a single base switch in the RBS: | ||
− | AAAGAGGAGAAA to AAAGAGGGGAAA | + | AAAGAGGAGAAA to AAAGAGGGGAAA. |
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− | [[Image:Q04401.JPG|thumb|500px|left|Transfer curve for Q04401 | + | [[Image:Q04401.JPG|thumb|500px|left|Transfer curve for Q04401 measured in the Screening Plasmid. Units are arbitrary. (<bbpart>pSB1A10</bbpart>)]] |
<br style="clear:both" /> | <br style="clear:both" /> | ||
Revision as of 07:48, 23 February 2008
TetR-derived transcriptional inverter
This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Transfer curve for Q04401 measured in the Screening Plasmid. Units are arbitrary. (pSB1A10)
Reuse
- This inverter was successfully reused by the [http://openwetware.org/wiki/IGEM:MIT/2006 2006 MIT iGEM team].
- Jasonk 02:44, 23 February 2008 (EST):In general, I think it's more likely to work when used on a high-copy (1 series) plasmid. I've tried it on a 3 series, using F2621 as the induction system and did not see flipping of the inverter.