Difference between revisions of "Part:BBa K2549007"
m
Line 3: Line 3:
 
<partinfo>BBa_K2549007 short</partinfo>
 
<partinfo>BBa_K2549007 short</partinfo>
  
−
This part is the mouse Notch1 extended regulatory region[1]. It can be served as the transmembrane core domain of the SynNotch.
+
This part is the mouse Notch1 extended regulatory region<ref>Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012</ref>. It was used as the transmembrane core domain of the SynNotch. However, original reported design has a high background activation in transfected 293T cells. We have put a lot of effect in optimizing this critical transmembrane domain for Notch receptor. Comparing with [[Part:BBa_K2549006]]
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2549007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2549007 SequenceAndFeatures</partinfo>
  
−
===References===
+
 
−
[1]Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012
+
<!-- Add more about the biology of this part here -->
 +
===Usage and Biology===
 +
TBA
 +
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2549007 parameters</partinfo>
 
<partinfo>BBa_K2549007 parameters</partinfo>
−
<!-- -->
+
-->
 +
 
 +
 
 +
===References===

Revision as of 03:12, 7 October 2018


mouse Notch1 core (extended)

This part is the mouse Notch1 extended regulatory region[1]. It was used as the transmembrane core domain of the SynNotch. However, original reported design has a high background activation in transfected 293T cells. We have put a lot of effect in optimizing this critical transmembrane domain for Notch receptor. Comparing with Part:BBa_K2549006


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 703


Usage and Biology

TBA



References

  1. ↑ Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, ..., Thomson M, Lim WA. Cell, 2016 Feb;164(4):780-91 PMID: 26830878; DOI: 10.1016/j.cell.2016.01.012