Difference between revisions of "Part:BBa K2886007"

Revision as of 07:41, 7 October 2018

VEGF-His9

VEGF (vascular endothelial growth factor) is a common ligand used in synthetic biology. And there are a lot of types of VEGF in human and rice like VEGFA-121, VEGFA-165 and so on. And in human, there are two main types of VEGFA: VEGF-121 and VEGFA-165.

VEGFA-121 was firstly introduced to iGEM by iGEM12_Slovenia in 2012, which was from mouse. In 2017, iGEM17_SYSU-CHINA improved this part by isolating it from the initial composite part and submitting the counterpart in human, making VEGFA-121 more mobile and suitable for human cell lines.

VEGFA-165 also was firstly introduced to iGEM by iGEM12_NYMU_Taiwan in 2012(BBa_K896922). However, there is no detail in the part wiki at all, and we actually find this part through blast tools. This year, Fudan-CHINA makes new improvements to VEGFA to make it more useful and enrich the chioces of iGEMers.

According to literatures, VEGFA-165 has a higher affinity to heparin and might has higher activity than VEGFA-121. Besides, VEGFA-165 is much more adopted in research compared with VEGFA-121. And what's more, buying VEGFA-165 from companies might be very expensive, so we add a His tag that could help us to express and purify VEGFA-165 easily. And We did succeed in collecting purified VEGFA-165 with the part we constructed, proving its reliability. What is also worth mentioning is that we could also us GS tag, but given it's even longer than VEGF itself, we turned to His tag ultimately.

All in all, through our work, VEGFA becomes much more available and easy to use.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 166

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 <partinfo>BBa_K2886007 short</partinfo>
-VEGF (vascular endothelial growth factor) is common ligand used in synthetic biology. And there are a lot of types of VEGF like VEGFA-121, VEGFA-165 and so on. VEGFA-121 was firstly introduced to iGEM by  iGEM12_Slovenia in 2012, which was from mouse. In 2017, iGEM17_SYSU-CHINA improved this part by isolating it from the initial composite part and submitting the counterpart in human.making VEGFA-121 more mobile and suitable for human cell lines.
+VEGF (vascular endothelial growth factor) is a common ligand used in synthetic biology. And there are a lot of types of VEGF in human and rice like VEGFA-121, VEGFA-165 and so on. And in human, there are two main types of VEGFA: VEGF-121 and VEGFA-165.
-This year, Fudan-CHINA makes new improvements to VEGFA to make it more useful. In Human there are two main types of VEGFA: VEGF-121 and VEGFA-165. Former teams introduced VEGFA-121 to iGEM and we this time we introduced another type VEGFA-165 to iGEM to enrich the chioces of iGEMers. According to literatures, VEGFA-165 has a higher affinity to heparin and might has higher activity than VEGFA-121. And VEGFA-165 is much more adopted by researcher in research.And what's more, Buying VEGFA-165 from companies might be very expensive, so we add a His tag that could help us to express and purify VEGFA-165 easily.And We did succeed in collecting purified VEGFA-165 with the part we constructed. What is also worth mentioning is that we could also us GS tag, but given it's even longer than VEGF itself, we turned to His tag ultimately.
+VEGFA-121 was firstly introduced to iGEM by  iGEM12_Slovenia in 2012, which was from mouse. In 2017, iGEM17_SYSU-CHINA improved this part by isolating it from the initial composite part and submitting the counterpart in human, making VEGFA-121 more mobile and suitable for human cell lines.
+VEGFA-165 also was firstly introduced to iGEM by  iGEM12_NYMU_Taiwan in 2012(BBa_K896922). However, there is no detail in the part wiki at all, and we actually find this part through blast tools. This year, Fudan-CHINA makes new improvements to VEGFA to make it more useful  and enrich the chioces of iGEMers.
+According to literatures, VEGFA-165 has a higher affinity to heparin and might has higher activity than VEGFA-121. Besides, VEGFA-165 is much more adopted in research compared with VEGFA-121. And what's more, buying VEGFA-165 from companies might be very expensive, so we add a His tag that could help us to express and purify VEGFA-165 easily. And We did succeed in collecting purified VEGFA-165 with the part we constructed, proving its reliability. What is also worth mentioning is that we could also us GS tag, but given it's even longer than VEGF itself, we turned to His tag ultimately.
 All in all, through our work, VEGFA becomes much more available and easy to use.