Difference between revisions of "Part:BBa K2549003:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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When fused with LaG2 using a flexible glycine-rich peptide linker to form a dimerization, ultra-high affinity can be conducted[1].
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Two copies of LaG16([[Part:Part:BBa_K2549002]]) were fused to form LaG2 using a flexible glycine-rich peptide linker<ref>A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170</ref>.
  
 
===Source===
 
===Source===
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IDT(gBlock)
 
IDT(gBlock)
  
 
===References===
 
===References===
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[1]A robust pipeline for rapid production of versatile nanobody repertoires.
 
−
Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP.
 
−
Nat Methods, 2014 Dec;11(12):1253-60  PMID: 25362362; DOI: 10.1038/nmeth.3170
 

Revision as of 02:56, 7 October 2018


anti-GFP (LaG16)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 243
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two copies of LaG16(Part:Part:BBa_K2549002) were fused to form LaG2 using a flexible glycine-rich peptide linker[1].

Source

IDT(gBlock)

References

  1. ↑ A robust pipeline for rapid production of versatile nanobody repertoires. Fridy PC, Li Y, Keegan S, ..., Chait BT, Rout MP. Nat Methods, 2014 Dec;11(12):1253-60 PMID: 25362362; DOI: 10.1038/nmeth.3170