Difference between revisions of "Part:BBa K2762015"

Revision as of 07:45, 6 October 2018

P_gadA-B0034-GFP

Characterization

This composite part includes a P_gadA (K1231001), a RBS (B0034) and a sfGFP (K2762014).Promoter gadA is a pH sensitive promoter from E. coli and gadA is one of the enzyme which is from glutamate decarboxylase (GAD) system. The expression of all of the gad genes in E. coli, including gadA is rely on the alternate sigma factor which encoded by rpoS, and repressed by the cAMP receptor protein and H-NS.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 937

Revision as of 07:44, 6 October 2018 (view source) PatrickChong (Talk \| contribs) ← Older edit		Revision as of 07:45, 6 October 2018 (view source) PatrickChong (Talk \| contribs) Newer edit →
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	===Characterization===		===Characterization===
−	This composite part includes a <i>P<sub>gadA</sub></i> (K1231001), a <i>RBS</i> (B0034) and a <i>sfGFP</i> (K2762014).Promoter gadA is a pH sensitive promoter from <i>E. coli</i>. <i>gadA</i> is one of the enzyme which is from glutamate decarboxylase (GAD) system. The expression of all of the <i>gad</i> genes in <i>E. coli</i>, including <i>gadA</i> is rely on the alternate sigma factor which encoded by <i>rpoS</i>, and repressed by the cAMP receptor protein and H-NS.	+	This composite part includes a <i>P<sub>gadA</sub></i> (K1231001), a <i>RBS</i> (B0034) and a <i>sfGFP</i> (K2762014).Promoter gadA is a pH sensitive promoter from <i>E. coli</i> and <i>gadA</i> is one of the enzyme which is from glutamate decarboxylase (GAD) system. The expression of all of the <i>gad</i> genes in <i>E. coli</i>, including <i>gadA</i> is rely on the alternate sigma factor which encoded by <i>rpoS</i>, and repressed by the cAMP receptor protein and H-NS.