Difference between revisions of "Part:BBa K2762015"
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===Characterization=== | ===Characterization=== | ||
− | This composite part includes a P<sub>gadA</sub> (K1231001), <i>RBS</i> (B0034) and a <i>sfGFP</i> (K2762014).Promoter gadA is a pH sensitive promoter from <i>E. coli</i>. It is one of the promoter which is from glutamate decarboxylase (GAD) system. | + | This composite part includes a <i>P<sub>gadA</sub></i> (K1231001), a <i>RBS</i> (B0034) and a <i>sfGFP</i> (K2762014).Promoter gadA is a pH sensitive promoter from <i>E. coli</i>. It is one of the promoter which is from glutamate decarboxylase (GAD) system. The expression of all of the <i>gad</i> genes in <i>E. coli</i>, including <i>gadA</i> is rely on the alternate sigma factor which encoded by <i>rpoS</i>, and repressed by the cAMP receptor protein and H-NS |
Revision as of 07:41, 6 October 2018
PgadA-B0034-GFP
Characterization
This composite part includes a PgadA (K1231001), a RBS (B0034) and a sfGFP (K2762014).Promoter gadA is a pH sensitive promoter from E. coli. It is one of the promoter which is from glutamate decarboxylase (GAD) system. The expression of all of the gad genes in E. coli, including gadA is rely on the alternate sigma factor which encoded by rpoS, and repressed by the cAMP receptor protein and H-NS
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 937