Difference between revisions of "Part:BBa K2593008"
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This part is a functional composite part, It consists of a P43 promotor, an RBS, and an operon of tuaD-gtaB which encode UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase, respectively, to participate in the biosynthesis of HA precursor UDP- GlcUA.
 
This part is a functional composite part, It consists of a P43 promotor, an RBS, and an operon of tuaD-gtaB which encode UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase, respectively, to participate in the biosynthesis of HA precursor UDP- GlcUA.
 
   
 
   
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<b>RBS(<a href="https://parts.igem.org/Part:BBa_K2593005">BBa_K2593005</a>)</b>: A strong ribosome binding site commonly used in Bacillus species, it is part of the pP43NMK shuttle vector.
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<b>RBS(<a href="https://parts.igem.org/Part:BBa_K2593005">BBa_K2593005</a>)</b>: A strong ribosome binding site commonly used in Bacillus species, it is part of the pP43NMK shuttle vector.<br>
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Promoter P43(BBa_K1628006)  
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Promoter P43(<a href="https://parts.igem.org/Part:BBa_K1628006">BBa_K1628006</a>):P43 is a constitutive promoter that common use in Bacillus subtilis168.<br>
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P43 is a constitutive promoter that common use in Bacillus subtilis168
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<b>tuaD(<a href="https://parts.igem.org/Part:BBa_K1469002">BBa_K1469002</a>)</b>: tuaD is one of native gene of B. subtilis, it encodes  UDP-glucose 6-dehydrogenase has 461 amino acid (also know as: UDP-GlcDH in megaterium).<br>
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<b>tuaD(<a href="https://parts.igem.org/Part:BBa_K1469002">BBa_K1469002</a>)</b>: tuaD is one of native gene of B. subtilis, it encodes  UDP-glucose 6-dehydrogenase has 461 amino acid (also know as: UDP-GlcDH in megaterium)
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<b>gtaB(<a href="https://parts.igem.org/Part:BBa_K1469005">BBa_K1469005</a>)</b>: gtaB gene encodes UTP--glucose-1-phosphate uridylyltransferase in Bacillus megaterium. The enzyme catalyzes the conversion of glucose 1-phospahate to UDP-glucose 1-phosphate.<br>
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<b>gtaB(<a href="https://parts.igem.org/Part:BBa_K1469005">BBa_K1469005</a>)</b>: gtaB gene encodes UTP--glucose-1-phosphate uridylyltransferase in Bacillus megaterium. The enzyme catalyzes the conversion of glucose 1-phospahate to UDP-glucose 1-phosphate.
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<b>T1 terminator(<a href="https://parts.igem.org/Part:BBa_B0010">BBa_B0010</a>)</b>:it is the most used terminator in bacteria. <br>
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<b>T1 terminator(<a href="https://parts.igem.org/Part:BBa_B0010">BBa_B0010</a>)</b>:it is the most used terminator in bacteria.  
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Revision as of 03:54, 6 October 2018

_NOTOC__ PlepA-RBS-AmyX -6His-LHyal-T1

This part is a functional composite part, It consists of a P43 promotor, an RBS, and an operon of tuaD-gtaB which encode UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase, respectively, to participate in the biosynthesis of HA precursor UDP- GlcUA. RBS(BBa_K2593005): A strong ribosome binding site commonly used in Bacillus species, it is part of the pP43NMK shuttle vector.
Promoter P43(BBa_K1628006):P43 is a constitutive promoter that common use in Bacillus subtilis168.
tuaD(BBa_K1469002): tuaD is one of native gene of B. subtilis, it encodes UDP-glucose 6-dehydrogenase has 461 amino acid (also know as: UDP-GlcDH in megaterium).
gtaB(BBa_K1469005): gtaB gene encodes UTP--glucose-1-phosphate uridylyltransferase in Bacillus megaterium. The enzyme catalyzes the conversion of glucose 1-phospahate to UDP-glucose 1-phosphate.
T1 terminator(BBa_B0010):it is the most used terminator in bacteria.

T Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 561


Reference