Difference between revisions of "Part:BBa K2804005:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The sequence of sfGFP was obtained from the Team Imperial (2014).
+
3A assembly
  
 
===References===
 
===References===

Revision as of 06:27, 9 October 2018


CBD cipA fused to sfGFP under the control of LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
    Illegal AgeI site found at 1493
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was neccesary to run a PCR to add the basepairs 5'-ccg-3'and 5'-aaaac-3 to the N-terminus and C-terminus of the gBlock in order to allow the enzymatic cleavage by EcoRI and PstI.


Source

3A assembly

References