Difference between revisions of "Part:BBa K2609026"

Revision as of 21:38, 7 October 2018

Lambda Red Recombinases under T7 expression system

This part generates the three Lambda ReD recombinases - exo, gam, beta - when expressed in a T7 expression strain like E. coli BL21(DE3).

Sequence and Features

Assembly Compatibility:

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 ===Usage and Biology===
+<h2> Biology </h2>
+<p>
+    The genes exo, gam and beta are a part of Bacteriophage Lambda's genome and is used in recomibination of bacteriophage DNA. The gam protein binds to the RecBCD nuclease of the host, thus protecting linear viral DNA from degradation. The protein exo is a 5' to 3' exonuclease which exposes the ends of linear dsDNA. The protein beta promotes single strand annealing and hence promotes homologous recombination. It is also important in rollin circle DNA replication which comes late in the infective cycle of the lambda phage.
+</p>
+<h2>Usage</h2>
+<h3> IISc-Bangalore iGEM 2018</h3>
+<p>
+    We used the this Lambda RED recombination system with a T4 Endolysin gene (<a href="https://parts.igem.org/Part:BBa_K2609017">BBa_K2609017</a>) to recombine Bacteriophage T4 and create a lysis deficient phage. This lysis deficient phage triggers its host to produce a monocyte chemoattractant <i>mcp-1</i> (<a href="https://parts.igem.org/Part:BBa_K2609000">BBa_K2609000</a>).
+</p>
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 <span class='h3bb'>Sequence and Features</span>