Difference between revisions of "User:Msicchio"
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== Punch tool cleaning protocol == | == Punch tool cleaning protocol == | ||
| − | Pull the punch tool apart. | + | Pull the punch tool apart, so that the black rod comes out of the column. |
Dip both the rod and the column briefly into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and a fourth bath of 95% ethanol. Ethanol is preferred as it dries much faster. Strong detergents should also be avoided. | Dip both the rod and the column briefly into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and a fourth bath of 95% ethanol. Ethanol is preferred as it dries much faster. Strong detergents should also be avoided. | ||
| − | Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up inside the column of the punch tool | + | Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up inside the column of the punch tool. It is important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency. |
== TE Buffer for thesis paper punches== | == TE Buffer for thesis paper punches== | ||
Revision as of 18:07, 15 February 2008
Punch tool cleaning protocol
Pull the punch tool apart, so that the black rod comes out of the column.
Dip both the rod and the column briefly into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and a fourth bath of 95% ethanol. Ethanol is preferred as it dries much faster. Strong detergents should also be avoided.
Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up inside the column of the punch tool. It is important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency.
TE Buffer for thesis paper punches
100 mmol Tris
5.1 mmol EDTA
can store paper either open or folded