Difference between revisions of "Part:BBa K2637042"

 
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<partinfo>BBa_K2637042 short</partinfo>
 
<partinfo>BBa_K2637042 short</partinfo>
  
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We constructed this pRS416 plasmid which contained two cassettes consisting of Gal1 promoter with mCherry and ADH1T terminator and Gal2 promoter with NanoLuc and CYC1 terminator by harnessing the principles of yeast homologous recombination. And only if the reporter genes function normally can we ensure that the system we reconstructed in the Saccharomyces cerevisiae succeeds.
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We constructed this pRS416 plasmid which contained two cassettes consisting of Gal1 promoter with mCherry and ADH1T terminator and Gal2 promoter with NanoLuc and CYC1 terminator by harnessing the principles of yeast homologous recombination. The plasmid incorporating these two cassettes which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. In detail, we built up the core oscillation system in the Saccharomyces cerevisiae, which originally can’t be directly observed or detected, but this oscillation information could be exhibited by the visible data images by utilizing the yeast two-hybrid system. In consideration of the yeast two-hybrid system which is prone to having false positive, we constructed this plasmid to minimize this phenomenon, allowing our conclusions to be more reliable. Only the data images embodied that the genes of mCherry and NanoLuc had been synchronously oscillating expressed can we ensure that our oscillation system definitely operated.
  
 
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Revision as of 05:11, 14 October 2018


Gal1 promoter+mCherry+ADH1 terminator+Gal2 promoter+NanoLuc+CYC1 terminator

We constructed this pRS416 plasmid which contained two cassettes consisting of Gal1 promoter with mCherry and ADH1T terminator and Gal2 promoter with NanoLuc and CYC1 terminator by harnessing the principles of yeast homologous recombination. The plasmid incorporating these two cassettes which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. In detail, we built up the core oscillation system in the Saccharomyces cerevisiae, which originally can’t be directly observed or detected, but this oscillation information could be exhibited by the visible data images by utilizing the yeast two-hybrid system. In consideration of the yeast two-hybrid system which is prone to having false positive, we constructed this plasmid to minimize this phenomenon, allowing our conclusions to be more reliable. Only the data images embodied that the genes of mCherry and NanoLuc had been synchronously oscillating expressed can we ensure that our oscillation system definitely operated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2890
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2098