Difference between revisions of "User:Msicchio"
(→Punch tool cleaning protocol) |
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Pull the punch tool apart. | Pull the punch tool apart. | ||
| − | Dip three times into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and 95% ethanol. | + | Dip three times into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and 95% ethanol. Ethanol dries much faster and Strong detergents should be avoided. |
| − | Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up into the punch tool, so it is best to blot It is important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency. | + | Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up into the punch tool, so it is best to blot It is also important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency. |
== TE Buffer for thesis paper punches== | == TE Buffer for thesis paper punches== | ||
Revision as of 17:13, 15 February 2008
Punch tool cleaning protocol
Pull the punch tool apart.
Dip three times into solutions of 10% bleach, distilled water, followed by second bath of distilled water, and 95% ethanol. Ethanol dries much faster and Strong detergents should be avoided.
Blot with a Kim wipe and allow to dry for 10 min. Note that the ethanol can go up into the punch tool, so it is best to blot It is also important that the punch tool is completely dry before punching out another DNA spot, as the alcohol will effect the transformation efficiency.
TE Buffer for thesis paper punches
100 mmol Tris
5.1 mmol EDTA
can store paper either open or folded